Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions

The protocols described here efficiently direct human pluripotent stem cells (hPSCs) to functional cardiomyocytes in a completely defined, serum-free system by temporal modulation of regulators of canonical Wnt signaling. Appropriate temporal application of Gsk3 inhibitor followed by expression of β...

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Detalhes bibliográficos
Main Authors: Lian, Xiaojun, Zhang, Jianhua, Azarin, Samira M., Zhu, Kexian, Hazeltine, Laurie B., Bao, Xiaoping, Hsiao, Cheston, Kamp, Timothy J., Palecek, Sean P.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3612968/
https://ncbi.nlm.nih.gov/pubmed/23257984
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2012.150
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