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Engineering RNA Endonucleases with Customized Sequence Specificities
Specific cleavage of RNAs is critical for in vitro manipulation of RNA and for in vivo gene silencing. Here we engineer artificial site-specific RNA endonucleases (ASREs) to function analogously to DNA restriction enzymes. We combine a general RNA cleavage domain with a series of Pumilio/FBF (PUF) d...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3612931/ https://ncbi.nlm.nih.gov/pubmed/23093184 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms2154 |
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