Use of a HpaII-polymerase chain reaction assay to study DNA methylation in the Pgk-1 CpG island of mouse embryos at the time of X-chromosome inactivation.

A HpaII-PCR assay was used to study DNA methylation in individual mouse embryos. It was found that HpaII site H-7 in the CpG island of the X-chromosome-linked Pgk-1 gene is less than or equal to 10% methylated in oocytes and male embryos but becomes 40% methylated in female embryos at 6.5 days; abou...

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Bibliographic Details
Main Authors: Singer-Sam, J, Grant, M, LeBon, J M, Okuyama, K, Chapman, V, Monk, M, Riggs, A D
Format: Artigo
Language:Inglês
Published: 1990
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC361130/
https://ncbi.nlm.nih.gov/pubmed/1697035
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