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In vivo phosphorylation dynamics of the Bordetella pertussis virulence-controlling response regulator BvgA

We have used protein electrophoresis through polyacrylamide gels derivatized with the proprietary ligand Phos-tag™ to separate the response regulator BvgA from its phosphorylated counterpart BvgA~P. This approach has allowed us to readily ascertain the degree of phosphorylation of BvgA in in vitro r...

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Detalhes bibliográficos
Main Authors: Boulanger, Alice, Chen, Qing, Hinton, Deborah M., Stibitz, Scott
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3608721/
https://ncbi.nlm.nih.gov/pubmed/23489959
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/mmi.12177
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