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In vivo phosphorylation dynamics of the Bordetella pertussis virulence-controlling response regulator BvgA
We have used protein electrophoresis through polyacrylamide gels derivatized with the proprietary ligand Phos-tag™ to separate the response regulator BvgA from its phosphorylated counterpart BvgA~P. This approach has allowed us to readily ascertain the degree of phosphorylation of BvgA in in vitro r...
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| Autors principals: | , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2013
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3608721/ https://ncbi.nlm.nih.gov/pubmed/23489959 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/mmi.12177 |
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