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Chlamydia Protease-Like Activity Factor (CPAF): Characterization of Proteolysis Activity In Vitro and Development of a Nanomolar Affinity CPAF Zymogen-Derived Inhibitor

During infection of epithelial cells, the obligate intracellular pathogen Chlamydia trachomatis secretes the serine protease CPAF into the host cytosol to regulate a range of host cellular processes through targeted proteolysis. Here we report the development of an in vitro assay for the enzyme and...

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Autores principales: Bednar, Maria M., Jorgensen, Ine, Valdivia, Raphael H., McCafferty, Dewey G.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2011
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3608462/
https://ncbi.nlm.nih.gov/pubmed/21830778
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi201098r
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