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Expression of a proteolipid gene from a high-copy-number plasmid confers trifluoperazine resistance to Saccharomyces cerevisiae.

A wild-type haploid yeast strain was transformed with a library of wild-type yeast DNA fragments ligated into a high-copy-number plasmid vector (YEp24). The pooled URA+ transformants were plated on rich medium containing a lethal concentration of trifluoperazine (TFP). Plasmids rescued into Escheric...

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Hlavní autoři: Shih, C K, Kwong, J, Montalvo, E, Neff, N
Médium: Artigo
Jazyk:Inglês
Vydáno: 1990
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC360771/
https://ncbi.nlm.nih.gov/pubmed/2192255
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