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A mutant screening method by critical annealing temperature-PCR for site-directed mutagenesis
BACKGROUND: Distinguishing desired mutants from parental templates and undesired mutants is a problem not well solved in Quikchange™ mutagenesis. Although Dpn I digestion can eliminate methylated parental (WT) DNA, the efficiency is not satisfying due to the existence of hemi-methylated DNA in the P...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3606131/ https://ncbi.nlm.nih.gov/pubmed/23497028 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-13-21 |
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