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Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms
We established a conditional site–specific recombination system based on dimerizable Cre–mediated recombination in the apicomplexan parasite Toxoplasma gondii. Using a novel single vector strategy that allows ligand-dependent, efficient removal of a gene of interest, we generated three knockouts of...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2012
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3605914/ https://ncbi.nlm.nih.gov/pubmed/23263690 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2301 |
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