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Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms

We established a conditional site–specific recombination system based on dimerizable Cre–mediated recombination in the apicomplexan parasite Toxoplasma gondii. Using a novel single vector strategy that allows ligand-dependent, efficient removal of a gene of interest, we generated three knockouts of...

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Bibliografiske detaljer
Main Authors: Andenmatten, Nicole, Egarter, Saskia, Jackson, Allison J, Jullien, Nicolas, Herman, Jean-Paul, Meissner, Markus
Format: Artigo
Sprog:Inglês
Udgivet: 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3605914/
https://ncbi.nlm.nih.gov/pubmed/23263690
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2301
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