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Separation of dansylated 17β-estradiol, 17α-estradiol and estrone on a single HPLC column for simultaneous quantitation by LC-MS/MS

We show here that baseline separation of dansylated estrone, 17β-estradiol and 17α-estradiol can be done, contrary to previous reports, within a short run time on a single RP-LC analytical column packed with particles bonded with phenyl-hexyl stationary phase. The chromatographic method coupled with...

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Autors principals: Szarka, Szabolcs, Nguyen, Vien, Prokai, Laszlo, Prokai-Tatrai, Katalin
Format: Artigo
Idioma:Inglês
Publicat: 2013
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3600063/
https://ncbi.nlm.nih.gov/pubmed/23371528
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-013-6710-y
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