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Reversible impairment of the ku80 gene by a recyclable marker in Aspergillus aculeatus

Auxotrophic mutants of Aspergillus can be isolated in the presence of counter-selective compounds, but the process is laborious. We developed a method to enable reversible impairment of the ku80 gene (Aaku80) in the imperfect fungus Aspergillus aculeatus. Aaku80 was replaced with a selection marker,...

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Detalhes bibliográficos
Main Authors: Tani, Shuji, Tsuji, Atsushi, Kunitake, Emi, Sumitani, Jun-ichi, Kawaguchi, Takashi
Formato: Artigo
Idioma:Inglês
Publicado em: Springer 2013
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3598690/
https://ncbi.nlm.nih.gov/pubmed/23311774
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/2191-0855-3-4
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