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Isolation of Human Eosinophils

Highly purified eosinophils can be isolated from peripheral blood by negative selection using an antibody-based magnetic negative selection protocol. The basic protocol describes a sequential fractionation of peripheral blood in which CD16+ granulocytes are enriched first from whole blood, followed...

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Main Authors: Akuthota, Praveen, Shamri, Revital, Weller, Peter F.
Formato: Artigo
Idioma:Inglês
Publicado: 2012
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3594798/
https://ncbi.nlm.nih.gov/pubmed/22855360
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471142735.im0731s98
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