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Single-shot proteomics using capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry produces more than 1,250 E. coli peptide identifications in a 50-minute separation

Capillary zone electrophoresis (CZE)-electrospray ionization-tandem mass spectrometry (ESI-MS/MS) was optimized and applied for analysis of 1–100 ng E. coli protein digests in a single run (single-shot analysis). The system employed an electrokinetically-pumped nanospray interface, a coated capillar...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Zhu, Guijie, Sun, Liangliang, Yan, Xiaojing, Dovichi, Norman J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3594080/
https://ncbi.nlm.nih.gov/pubmed/23394296
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac303750g
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