Cloning, Expression, and in vitro Functional Activity Assay of phiC31 Integrase cDNA in Escherichia coli

OBJECTIVE: The aim of present study was cloning and expression of phiC31 integrase cDNA in a bacterial expression vector. Thus, an intra molecular assay vector was applied to show in vitro activity of recombinant protein. MATERIALS AND METHODS: In this experimental study, phiC31 cDNA was subcloned i...

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Detalhes bibliográficos
Main Authors: Sekhavati, Mohammad Hadi, Tahmoorespur, Mojtaba, Ghaedi, Kamran, Dormiani, Kianoush, Nassiri, Mohammad Reza, Khazaie, Yahya, Foruzanfar, Mahboubeh, Hosseini, Morteza, Nasr Esfahani, Mohammad Hossein
Formato: Artigo
Idioma:Inglês
Publicado em: Royan Institute 2013
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3593930/
https://ncbi.nlm.nih.gov/pubmed/23577305
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