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RNA Preparation of Saccharomyces cerevisiae Using the Digestion Method may Give Misleading Results

Zymolyase (lyticase) is used for cell wall digestion in yeast experiments and is needed for incubation processes under moderate experimental conditions. This has been thought to cause unfavorable effects, and many researchers are aware that the enzyme method is unsuitable for RNA preparation followi...

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Autors principals: Suzuki, Tadahiro, Iwahashi, Yumiko
Format: Artigo
Idioma:Inglês
Publicat: Springer-Verlag 2013
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3593083/
https://ncbi.nlm.nih.gov/pubmed/23325148
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12010-012-0051-8
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