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Hierarchical molecular tagging to resolve long continuous sequences by massively parallel sequencing
Here we demonstrate the use of short-read massive sequencing systems to in effect achieve longer read lengths through hierarchical molecular tagging. We show how indexed and PCR-amplified targeted libraries are degraded, sub-sampled and arrested at timed intervals to achieve pools of differing avera...
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| Hlavní autoři: | , , , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Nature Publishing Group
2013
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3592332/ https://ncbi.nlm.nih.gov/pubmed/23470464 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep01186 |
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