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Efficient Production of Active Polyhydroxyalkanoate Synthase in Escherichia coli by Coexpression of Molecular Chaperones

The type I polyhydroxyalkanoate synthase from Cupriavidus necator was heterologously expressed in Escherichia coli with simultaneous overexpression of chaperone proteins. Compared to expression of synthase alone (14.55 mg liter(−1)), coexpression with chaperones resulted in the production of larger...

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書誌詳細
主要な著者: Thomson, Nicholas M., Saika, Azusa, Ushimaru, Kazunori, Sangiambut, Smith, Tsuge, Takeharu, Summers, David K., Sivaniah, Easan
フォーマット: Artigo
言語:Inglês
出版事項: American Society for Microbiology 2013
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC3592256/
https://ncbi.nlm.nih.gov/pubmed/23335776
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02881-12
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