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Influence of Temperature, Medium, and Storage Duration on Chlamydia trachomatis DNA Detection by PCR

We validated the use of stored samples for Chlamydia trachomatis research. C. trachomatis DNA was detected by real-time PCR in clinical (urine and self-taken vaginal swabs) and spiked samples using six different media, five different time points (up to 2 years), and four different temperature condit...

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Main Authors: van Dommelen, Laura, Wolffs, Petra F. G., van Tiel, Frank H., Dukers, Nicole, Herngreen, Selma B., Bruggeman, Cathrien A., Hoebe, Christian J. P. A.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2013
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3592091/
https://ncbi.nlm.nih.gov/pubmed/23254134
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02631-12
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