Expression, purification and characterization of a recombinant fusion protein based on the human papillomavirus-16 E7 antigen

A fusion protein comprising a cell penetrating and immunostimulatory peptide corresponding to residues 32 to 51 of the Limulus polyphemus protein linked to human papillomavirus (HPV)-16 E7 antigen (LALF(32-51)-E7) was expressed in E. coli BL21 (DE3) cells. The recombinant protein in E. coli accounte...

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Bibliografske podrobnosti
Main Authors: Granadillo, Milaid, Batte, Aileen, Lugo, Victoria M, Musacchio, Alexis, Bequet-Romero, Mónica, Betancourt, Lázaro, Besada, Vladimir, Javier, Luis, Molina, Raychel, Falcón, Viviana, Torrens, Isis
Format: Artigo
Jezik:Inglês
Izdano: Springer International Publishing AG 2013
Teme:
Research
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3590404/
https://ncbi.nlm.nih.gov/pubmed/23483788
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/2193-1801-2-12
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