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Characterization of the kinetics of RNA annealing and strand displacement activities of the E. coli DEAD-box helicase CsdA

CsdA is one of five E. coli DEAD-box helicases and as a cold-shock protein assists RNA structural remodeling at low temperatures. The helicase has been shown to catalyze duplex unwinding in an ATP-dependent way and accelerate annealing of complementary RNAs, but detailed kinetic analyses are missing...

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Main Authors: Stampfl, Sabine, Doetsch, Martina, Beich-Frandsen, Mads, Schroeder, Renée
Format: Artigo
Jezik:Inglês
Izdano: Landes Bioscience 2013
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3590231/
https://ncbi.nlm.nih.gov/pubmed/23291905
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/rna.23475
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