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Expression and Purification of Functional Human Mu Opioid Receptor from E.coli

N-terminally his-tagged human mu opioid receptor, a G protein-coupled receptor was produced in E.coli employing synthetic codon-usage optimized constructs. The receptor was expressed in inclusion bodies and membrane-inserted in different E.coli strains. By optimizing the expression conditions the ex...

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Detalhes bibliográficos
Main Authors: Ma, Yanbin, Kubicek, Jan, Labahn, Jörg
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3578875/
https://ncbi.nlm.nih.gov/pubmed/23437147
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0056500
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