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A simple photoactivation and image analysis module for visualizing and analyzing axonal transport with high temporal resolution
We describe a strategy for analyzing axonal transport of cytosolic proteins (cps) using photoactivatable GFp—paGFp—with modifications of standard imaging components that can be retroactively fitted to a conventional epifluorescence microscope. the photoactivation and visualization are nearly simulta...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2011
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3568933/ https://ncbi.nlm.nih.gov/pubmed/22179592 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.428 |
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