Rapid and Sensitive Detection of Mycobacterium avium subsp. paratuberculosis in Bovine Milk and Feces by a Combination of Immunomagnetic Bead Separation-Conventional PCR and Real-Time PCR

Immunomagnetic bead separation coupled with bead beating and real-time PCR was found to be a very effective procedure for the isolation, separation, and detection of Mycobacterium avium subsp. paratuberculosis from milk and/or fecal samples from cattle and American bison. Samples were spiked with M....

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Autors principals: Khare, Sangeeta, Ficht, Thomas A., Santos, Renato L., Romano, Juan, Ficht, Allison R., Zhang, Shuping, Grant, Irene R., Libal, Melissa, Hunter, David, Adams, L. Garry
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2004
Matèries:
Clinical Veterinary Microbiology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC356818/
https://ncbi.nlm.nih.gov/pubmed/15004056
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.3.1075-1081.2004
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