Nepenthesin from Monkey Cups for Hydrogen/Deuterium Exchange Mass Spectrometry

Studies of protein dynamics, structure and interactions using hydrogen/deuterium exchange mass spectrometry (HDX-MS) have sharply increased over the past 5–10 years. The predominant technology requires fast digestion at pH 2–3 to retain deuterium label. Pepsin is used almost exclusively, but it prov...

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Autors principals: Rey, Martial, Yang, Menglin, Burns, Kyle M., Yu, Yaping, Lees-Miller, Susan P., Schriemer, David C.
Format: Artigo
Idioma:Inglês
Publicat: The American Society for Biochemistry and Molecular Biology 2013
Matèries:
Research
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3567866/
https://ncbi.nlm.nih.gov/pubmed/23197791
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M112.025221
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