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Elimination of autofluorescence background from fluorescence tissue images by use of time-gated detection and the AzaDiOxaTriAngulenium (ADOTA) fluorophore

Sample autofluorescence (fluorescence of inherent components of tissue and fixative-induced fluorescence) is a significant problem in direct imaging of molecular processes in biological samples. A large variety of naturally occurring fluorescent components in tissue results in broad emission that ov...

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Detalhes bibliográficos
Main Authors: Rich, Ryan M., Stankowska, Dorota L., Maliwal, Badri P., Sørensen, Thomas Just, Laursen, Bo W., Krishnamoorthy, Raghu R., Gryczynski, Zygmunt, Borejdo, Julian, Gryczynski, Ignacy, Fudala, Rafal
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3566262/
https://ncbi.nlm.nih.gov/pubmed/23254457
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-012-6623-1
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