Crystallization, characterization and preliminary X-ray crystallographic analysis of GK2848, a putative carbonic anhydrase of Geobacillus kaustophilus

GK2848, a hypothetical protein from the thermophilic organism Geobacillus kaustophilus, was cloned and overexpressed in Escherichia coli. The protein was purified to homogeneity using Ni–NTA affinity-column and gel-filtration chromatography. The purified protein was crystallized using the sitting-dr...

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Main Authors: Ragunathan, Preethi, Raghunath, Gokul, Kuramitsu, Seiki, Yokoyama, Shigeyuki, Kumarevel, Thirumananseri, Ponnuraj, Karthe
Formato: Artigo
Idioma:Inglês
Publicado: International Union of Crystallography 2013
Assuntos:
Crystallization Communications
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3564620/
https://ncbi.nlm.nih.gov/pubmed/23385759
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112051913
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