Caulobacter chromosome in vivo configuration matches model predictions for a supercoiled polymer in a cell-like confinement

We measured the distance between fluorescent-labeled DNA loci of various interloci contour lengths in Caulobacter crescentus swarmer cells to determine the in vivo configuration of the chromosome. For DNA segments less than about 300 kb, the mean interloci distances, 〈r〉, scale as n(0.22), where n i...

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Autors principals: Hong, Sun-Hae, Toro, Esteban, Mortensen, Kim I., de la Rosa, Mario A. Díaz, Doniach, Sebastian, Shapiro, Lucy, Spakowitz, Andrew J., McAdams, Harley H.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2013
Matèries:
Biological Sciences
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3562846/
https://ncbi.nlm.nih.gov/pubmed/23319648
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1220824110
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