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Suppression of artifacts and barcode bias in high-throughput transcriptome analyses utilizing template switching

Template switching (TS) has been an inherent mechanism of reverse transcriptase, which has been exploited in several transcriptome analysis methods, such as CAGE, RNA-Seq and short RNA sequencing. TS is an attractive option, given the simplicity of the protocol, which does not require an adaptor med...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Tang, Dave T. P., Plessy, Charles, Salimullah, Md, Suzuki, Ana Maria, Calligaris, Raffaella, Gustincich, Stefano, Carninci, Piero
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3562004/
https://ncbi.nlm.nih.gov/pubmed/23180801
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks1128
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