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Conformationally Trapping the Actin-binding Cleft of Myosin with a Bifunctional Spin Label
We have trapped the catalytic domain of Dictyostelium (Dicty) myosin II in a weak actin-binding conformation by chemically crosslinking two engineered cysteines across the actin-binding cleft, using a bifunctional spin label (BSL). By connecting the lower and upper 50 kDa domains of myosin, the cros...
Tallennettuna:
| Päätekijät: | , , |
|---|---|
| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
American Society for Biochemistry and Molecular Biology
2013
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| Aiheet: | |
| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3561526/ https://ncbi.nlm.nih.gov/pubmed/23250750 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M112.428565 |
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