An efficient procedure for protein extraction from formalin-fixed, paraffin-embedded tissues for reverse phase protein arrays

INTRODUCTION: Protein extraction from formalin-fixed paraffin-embedded (FFPE) tissues is challenging due to extensive molecular crosslinking that occurs upon formalin fixation. Reverse-phase protein array (RPPA) is a high-throughput technology, which can detect changes in protein levels and protein...

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Detalhes bibliográficos
Main Authors: Guo, Huifang, Liu, Wenbin, Ju, Zhenlin, Tamboli, Pheroze, Jonasch, Eric, Mills, Gordon B, Lu, Yiling, Hennessy, Bryan T, Tsavachidou, Dimitra
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2012
Assuntos:
Methodology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3561137/
https://ncbi.nlm.nih.gov/pubmed/23006314
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1477-5956-10-56
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