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Residue-Specific Membrane Location of Peptides and Proteins Using Specifically and Extensively Deuterated Lipids and 13C-2H Rotational-Echo Double-Resonance Solid-State NMR

Residue-specific location of peptides in the hydrophobic core of membranes was examined using 13C-2H REDOR and samples in which the lipids were selectively deuterated. The transmembrane topology of the KALP peptide was validated with this approach with substantial dephasing observed for deuteration...

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Detalhes bibliográficos
Main Authors: Xie, Li, Ghosh, Ujjayini, Schmick, Scott D., Weliky, David P.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3557618/
https://ncbi.nlm.nih.gov/pubmed/23225071
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-012-9692-8
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