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New Method for Large-Scale Preparation of Covalently Closed λ DNA Molecules
A combination of mutations in bacteriophage λ and its host Escherichia coli K-12 provides a convenient system for the isolation of large quantities of covalently closed circular DNA molecules. We describe two procedures for the large scale preparation of λ DNA in the duplex circular form.
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| Autori principali: | , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
1974
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC355626/ https://ncbi.nlm.nih.gov/pubmed/4610176 |
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