Purification of DNA-origami nanostructures by rate-zonal centrifugation

Most previously reported methods for purifying DNA-origami nanostructures rely on agarose-gel electrophoresis (AGE) for separation. Although AGE is routinely used to yield 0.1–1 µg purified DNA nanostructures, obtaining >100 µg of purified DNA-origami structure through AGE is typically laborious...

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Bibliographic Details
Main Authors: Lin, Chenxiang, Perrault, Steven D., Kwak, Minseok, Graf, Franziska, Shih, William M.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2013
Subjects:
Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3553994/
https://ncbi.nlm.nih.gov/pubmed/23155067
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks1070
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