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Strand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selection

BACKGROUND: High throughput DNA sequencing technology has enabled quantification of all the RNAs in a cell or tissue, a method widely known as RNA sequencing (RNA-Seq). However, non-coding RNAs such as rRNA are highly abundant and can consume >70% of sequencing reads. A common approach is to extr...

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Detalhes bibliográficos
Main Authors: Zhang, Zhao, Theurkauf, William E, Weng, Zhiping, Zamore, Phillip D
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3552703/
https://ncbi.nlm.nih.gov/pubmed/23273270
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1758-907X-3-9
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