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Method to quantify live and dead cells in multi-species oral biofilm by real-time PCR with propidium monoazide

Real-time PCR (qPCR) is a widely used technique in analysing environmental and clinical microbiological samples. However, its main limitation was its inability to discriminate between live and dead cells. Recently, propidium monoazide (PMA) together with qPCR has been used to overcome this problem,...

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Main Authors: Àlvarez, Gerard, González, Marta, Isabal, Sergio, Blanc, Vanessa, León, Rubén
格式: Artigo
語言:Inglês
出版: Springer 2013
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3549832/
https://ncbi.nlm.nih.gov/pubmed/23289803
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/2191-0855-3-1
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