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STED Nanoscopy with Time-Gated Detection: Theoretical and Experimental Aspects

In a stimulated emission depletion (STED) microscope the region in which fluorescence markers can emit spontaneously shrinks with continued STED beam action after a singular excitation event. This fact has been recently used to substantially improve the effective spatial resolution in STED nanoscopy...

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Detalhes bibliográficos
Main Authors: Vicidomini, Giuseppe, Schönle, Andreas, Ta, Haisen, Han, Kyu Young, Moneron, Gael, Eggeling, Christian, Hell, Stefan W.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3548795/
https://ncbi.nlm.nih.gov/pubmed/23349884
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0054421
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