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Detection of adenovirus type 2-induced early polypeptides using cycloheximide pretreatment to enhance viral protein synthesis.

(35S) methionine-labeled polypeptides synthesized by adenovirus type 2-infected cells have been analyzed by polyacrylamide gradient gel electrophoresis and autoradiography. Cycloheximide (CH) was added to infected cultures to accumulate early viral mRNA relative to host cell mRNA. This allowed viral...

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Main Authors: Harter, M L, Shanmugam, G, Wold, W S, Green, M
Format: Artigo
Jezik:Inglês
Izdano: 1976
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC354851/
https://ncbi.nlm.nih.gov/pubmed/950686
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