Translation of T7 RNA in vitro without cleavage by RNase III.
T7 early mRNA's are generated from a high-molecular-weight precursor RNA by site-specific RNase III cleavage. When T7 DNA is transcribed in vitro by Escherichia coli RNA polymerase, the transcript is a large, single-piece RNA equivalent to the in vivo precursor RNA. The T7 RNA synthesized in vitro c...
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| Udgivet i: | J Virol |
|---|---|
| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1976
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC354818/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/775130/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.18.3.1155-1159.1976 |
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