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Identification of bacteriophage T4-specific precursor tRNA by using a host mutant defective in the methylation of tRNA.
A mutant of Escherichia coli K-12 that is defective in the synthesis of 5-methyluridine (ribothymidine) in tRNA was used to identify precursors to phage T4-specific tRNA. The precursor molecules, isolated by gel filtration, were more than twice the size of tRNA. This method is suitable for isolation...
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| Autor principal: | |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1975
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC354724/ https://ncbi.nlm.nih.gov/pubmed/1099234 |
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