Transfection of Escherichia coli spheroplasts. VI. Transfection of nonpermissive spheroplasts by T5 and BF23 bacteriophage DNA carrying amber mutations in DNA transfer genes.

DNA was extracted from T5 and BF23 phage carrying amber mutations in genes A2, A1, or D9 and tested for its ability to transfect su minus spheroplasts. DNA from T5 am231, defective in gene A2, transfects Escherichia coli su minus recB minus spheroplasts with an efficiency of 16% of that of wild-type...

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Bibliographic Details
Main Authors: Benzinger, R, McCorquodale, D J
Format: Artigo
Language:Inglês
Published: 1975
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC354625/
https://ncbi.nlm.nih.gov/pubmed/805845
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