Active Targeting to Osteosarcoma Cells and Apoptotic Cell Death Induction by the Novel Lectin Eucheuma serra Agglutinin Isolated from a Marine Red Alga

Previously, we demonstrated that the novel lectin Eucheuma serra agglutinin from a marine red alga (ESA) induces apoptotic cell death in carcinoma. We now find that ESA induces apoptosis also in the case of sarcoma cells. First, propidium iodide assays with OST cells and LM8 cells showed a decrease...

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Autores principales: Hayashi, Keita, Walde, Peter, Miyazaki, Tatsuhiko, Sakayama, Kenshi, Nakamura, Atsushi, Kameda, Kenji, Masuda, Seizo, Umakoshi, Hiroshi, Kato, Keiichi
Formato: Artigo
Lenguaje:Inglês
Publicado: Hindawi Publishing Corporation 2012
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Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3543805/
https://ncbi.nlm.nih.gov/pubmed/23346404
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2012/842785
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spelling pubmed-35438052013-01-23 Active Targeting to Osteosarcoma Cells and Apoptotic Cell Death Induction by the Novel Lectin Eucheuma serra Agglutinin Isolated from a Marine Red Alga Hayashi, Keita Walde, Peter Miyazaki, Tatsuhiko Sakayama, Kenshi Nakamura, Atsushi Kameda, Kenji Masuda, Seizo Umakoshi, Hiroshi Kato, Keiichi J Drug Deliv Research Article Previously, we demonstrated that the novel lectin Eucheuma serra agglutinin from a marine red alga (ESA) induces apoptotic cell death in carcinoma. We now find that ESA induces apoptosis also in the case of sarcoma cells. First, propidium iodide assays with OST cells and LM8 cells showed a decrease in cell viability after addition of ESA. With 50 μg/ml ESA, the viabilities after 24 hours decreased to 54.7 ± 11.4% in the case of OST cells and to 41.7 ± 12.3% for LM8 cells. Second, using fluorescently labeled ESA and flow cytometric and fluorescence microscopic measurements, it could be shown that ESA does not bind to cells that were treated with glycosidases, indicating importance of the carbohydrate chains on the surface of the cells for efficient ESA-cell interactions. Third, Span 80 vesicles with surface-bound ESA as active targeting ligand were shown to display sarcoma cell binding activity, leading to apoptosis and complete OST cell death after 48 hours at 2 μg/ml ESA. The findings indicate that Span 80 vesicles with surface-bound ESA are a potentially useful drug delivery system not only for the treatment of carcinoma but also for the treatment of osteosarcoma. Hindawi Publishing Corporation 2012 2012-12-27 /pmc/articles/PMC3543805/ /pubmed/23346404 http://dx.doi.org/10.1155/2012/842785 Text en Copyright © 2012 Keita Hayashi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
institution US NLM
collection PubMed Central
language Inglês
format Artigo
topic Research Article
spellingShingle Research Article
Hayashi, Keita
Walde, Peter
Miyazaki, Tatsuhiko
Sakayama, Kenshi
Nakamura, Atsushi
Kameda, Kenji
Masuda, Seizo
Umakoshi, Hiroshi
Kato, Keiichi
Active Targeting to Osteosarcoma Cells and Apoptotic Cell Death Induction by the Novel Lectin Eucheuma serra Agglutinin Isolated from a Marine Red Alga
description Previously, we demonstrated that the novel lectin Eucheuma serra agglutinin from a marine red alga (ESA) induces apoptotic cell death in carcinoma. We now find that ESA induces apoptosis also in the case of sarcoma cells. First, propidium iodide assays with OST cells and LM8 cells showed a decrease in cell viability after addition of ESA. With 50 μg/ml ESA, the viabilities after 24 hours decreased to 54.7 ± 11.4% in the case of OST cells and to 41.7 ± 12.3% for LM8 cells. Second, using fluorescently labeled ESA and flow cytometric and fluorescence microscopic measurements, it could be shown that ESA does not bind to cells that were treated with glycosidases, indicating importance of the carbohydrate chains on the surface of the cells for efficient ESA-cell interactions. Third, Span 80 vesicles with surface-bound ESA as active targeting ligand were shown to display sarcoma cell binding activity, leading to apoptosis and complete OST cell death after 48 hours at 2 μg/ml ESA. The findings indicate that Span 80 vesicles with surface-bound ESA are a potentially useful drug delivery system not only for the treatment of carcinoma but also for the treatment of osteosarcoma.
author Hayashi, Keita
Walde, Peter
Miyazaki, Tatsuhiko
Sakayama, Kenshi
Nakamura, Atsushi
Kameda, Kenji
Masuda, Seizo
Umakoshi, Hiroshi
Kato, Keiichi
author_facet Hayashi, Keita
Walde, Peter
Miyazaki, Tatsuhiko
Sakayama, Kenshi
Nakamura, Atsushi
Kameda, Kenji
Masuda, Seizo
Umakoshi, Hiroshi
Kato, Keiichi
author_sort Hayashi, Keita
title Active Targeting to Osteosarcoma Cells and Apoptotic Cell Death Induction by the Novel Lectin Eucheuma serra Agglutinin Isolated from a Marine Red Alga
title_short Active Targeting to Osteosarcoma Cells and Apoptotic Cell Death Induction by the Novel Lectin Eucheuma serra Agglutinin Isolated from a Marine Red Alga
title_full Active Targeting to Osteosarcoma Cells and Apoptotic Cell Death Induction by the Novel Lectin Eucheuma serra Agglutinin Isolated from a Marine Red Alga
title_fullStr Active Targeting to Osteosarcoma Cells and Apoptotic Cell Death Induction by the Novel Lectin Eucheuma serra Agglutinin Isolated from a Marine Red Alga
title_full_unstemmed Active Targeting to Osteosarcoma Cells and Apoptotic Cell Death Induction by the Novel Lectin Eucheuma serra Agglutinin Isolated from a Marine Red Alga
title_sort active targeting to osteosarcoma cells and apoptotic cell death induction by the novel lectin eucheuma serra agglutinin isolated from a marine red alga
publisher Hindawi Publishing Corporation
publishDate 2012
url https://ncbi.nlm.nih.gov/pmc/articles/PMC3543805/
https://ncbi.nlm.nih.gov/pubmed/23346404
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2012/842785
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