Modulation of mouse mammary tumor virus production in the MJY-alpha cell line.

Implantation of the mouse mammary tumor virus (MMTV)-producing mammary tumor cell line MJY-alpha into isogeneic mice elicited both humoral and T-cell responses against MMTV virion antigens. The carcinosarcomas which developed from the implanted cells showed a significant decrease in MMTV synthesis,...

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Main Authors: Yagi, M J, Blair, P B, Lane, M A
Format: Artigo
Sprog:Inglês
Udgivet: 1978
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC354309/
https://ncbi.nlm.nih.gov/pubmed/214582
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spelling pubmed-3543092004-03-04 Modulation of mouse mammary tumor virus production in the MJY-alpha cell line. Yagi, M J Blair, P B Lane, M A J Virol Research Article Implantation of the mouse mammary tumor virus (MMTV)-producing mammary tumor cell line MJY-alpha into isogeneic mice elicited both humoral and T-cell responses against MMTV virion antigens. The carcinosarcomas which developed from the implanted cells showed a significant decrease in MMTV synthesis, compared with cells remaining in culture, which was detectable as early as 7 days after implantation and for five transplant generations. Electron microscopic examination of thin sections of the tumors revealed that intracytoplasmic A particles, budding particles, and cell-free MMTV B particles were all affected. However, immunofluorescence assays of tumor sections demonstrated the presence of MMTV viral antigens in the cells. Cell cultures initiated from first-, third-, and fourth-generation tumors were morphologically identical to the original in vitro cell line, although virus production was barely detectable. Analysis of the cultures by electron microscopy revealed a significant increase in MMTV virions after in vitro passage 3. Polypeptide profiles obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of virions purified from these cultures were identical to MMTV. Immunodiffusion demonstrated the cross-reactivity between these virions and MMTV particles obtained from mouse milk. In vitro treatment of MJY-alpha cell cultures with rabbit anti-MMTV antiserum resulted in a reduction of extracellular MMTV virions, as well as alterations in their sodium dodecyl sulfate-polyacrylamide gel electrophoretic polypeptide patterns. 1978-11 /pmc/articles/PMC354309/ /pubmed/214582 Text en
institution US NLM
collection PubMed Central
language Inglês
format Artigo
topic Research Article
spellingShingle Research Article
Yagi, M J
Blair, P B
Lane, M A
Modulation of mouse mammary tumor virus production in the MJY-alpha cell line.
description Implantation of the mouse mammary tumor virus (MMTV)-producing mammary tumor cell line MJY-alpha into isogeneic mice elicited both humoral and T-cell responses against MMTV virion antigens. The carcinosarcomas which developed from the implanted cells showed a significant decrease in MMTV synthesis, compared with cells remaining in culture, which was detectable as early as 7 days after implantation and for five transplant generations. Electron microscopic examination of thin sections of the tumors revealed that intracytoplasmic A particles, budding particles, and cell-free MMTV B particles were all affected. However, immunofluorescence assays of tumor sections demonstrated the presence of MMTV viral antigens in the cells. Cell cultures initiated from first-, third-, and fourth-generation tumors were morphologically identical to the original in vitro cell line, although virus production was barely detectable. Analysis of the cultures by electron microscopy revealed a significant increase in MMTV virions after in vitro passage 3. Polypeptide profiles obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of virions purified from these cultures were identical to MMTV. Immunodiffusion demonstrated the cross-reactivity between these virions and MMTV particles obtained from mouse milk. In vitro treatment of MJY-alpha cell cultures with rabbit anti-MMTV antiserum resulted in a reduction of extracellular MMTV virions, as well as alterations in their sodium dodecyl sulfate-polyacrylamide gel electrophoretic polypeptide patterns.
author Yagi, M J
Blair, P B
Lane, M A
author_facet Yagi, M J
Blair, P B
Lane, M A
author_sort Yagi, M J
title Modulation of mouse mammary tumor virus production in the MJY-alpha cell line.
title_short Modulation of mouse mammary tumor virus production in the MJY-alpha cell line.
title_full Modulation of mouse mammary tumor virus production in the MJY-alpha cell line.
title_fullStr Modulation of mouse mammary tumor virus production in the MJY-alpha cell line.
title_full_unstemmed Modulation of mouse mammary tumor virus production in the MJY-alpha cell line.
title_sort modulation of mouse mammary tumor virus production in the mjy-alpha cell line.
publishDate 1978
url https://ncbi.nlm.nih.gov/pmc/articles/PMC354309/
https://ncbi.nlm.nih.gov/pubmed/214582
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