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Functional multiplex reporter assay using tagged Gaussia luciferase

We have developed a multiplex reporter system to monitor multiple biological variables in real-time. The secreted Gaussia luciferase was fused to ten different epitope tags (Gluc(tag)), each expressed in different tumor cells. By immunobinding of the tags followed by Gluc(tag) detection, this system...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: van Rijn, Sjoerd, Nilsson, Jonas, Noske, David P., Vandertop, W. Peter, Tannous, Bakhos A., Würdinger, Thomas
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3541509/
https://ncbi.nlm.nih.gov/pubmed/23308339
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep01046
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