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Purification and molecular characterization of adenovirus type 2 DNA-binding protein.

An adenovirus type 2 (Ad2) DNA-binding protein was purified by sequential DNA-cellulose, Sephadex G-200, and DEAE-Sephadex chromatography, with a yield of 120 mug of binding protein (95 to 99% homogeneity) starting with 2 X 10(9) infected cells. By omitting the Sephadex G-200 step, 400 to 600 mug of...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:J Virol
Hauptverfasser: Sugawara, K, Gilead, Z, Green, M
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1977
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC353820/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/64628/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.21.1.338-346.1977
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