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Construction of a specific amber codon in the simian virus 40 T-antigen gene by site-directed mutagenesis.

The site-directed bisulfite mutagenesis technique has been used to construct a specific mutation, am404, at nucleotide position 3124 in the simian virus 40 genome. The mutation was contained within a PstI restriction site (map position 0.27) and prevented cleavage by PstI at that position. Nucleotid...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:J Virol
Hauptverfasser: Rawlins, D R, Muzyczka, N
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1980
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC353682/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6253682/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.36.2.611-616.1980
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