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Construction of a specific amber codon in the simian virus 40 T-antigen gene by site-directed mutagenesis.
The site-directed bisulfite mutagenesis technique has been used to construct a specific mutation, am404, at nucleotide position 3124 in the simian virus 40 genome. The mutation was contained within a PstI restriction site (map position 0.27) and prevented cleavage by PstI at that position. Nucleotid...
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| Veröffentlicht in: | J Virol |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology (ASM)
1980
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC353682/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6253682/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.36.2.611-616.1980 |
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