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Assessment of Real-Time PCR Assay for Detection of Rickettsia spp. and Rickettsia rickettsii in Banked Clinical Samples

Two novel real-time PCR assays were developed for the detection of Rickettsia spp. One assay detects all tested Rickettsia spp.; the other is specific for Rickettsia rickettsii. Evaluation using DNA from human blood and tissue samples showed both assays to be more sensitive than nested PCR assays cu...

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Bibliographic Details
Main Authors: Kato, Cecilia Y., Chung, Ida H., Robinson, Lauren K., Austin, Amy L., Dasch, Gregory A., Massung, Robert F.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2013
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3536194/
https://ncbi.nlm.nih.gov/pubmed/23135935
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01723-12
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