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Analysis of regional brain mitochondrial bioenergetics and susceptibility to mitochondrial inhibition utilizing a microplate based system

The analysis of mitochondrial bioenergetic function typically has required 50–100 μg of protein per sample and at least 15 min per run when utilizing a Clark-type oxygen electrode. In the present work we describe a method utilizing the Seahorse Biosciences XF24 Flux Analyzer for measuring mitochondr...

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Autors principals: Sauerbeck, Andrew, Pandya, Jignesh, Singh, Indrapal, Bittman, Kevin, Readnower, Ryan, Bing, Guoying, Sullivan, Patrick
Format: Artigo
Idioma:Inglês
Publicat: 2011
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3535268/
https://ncbi.nlm.nih.gov/pubmed/21402103
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jneumeth.2011.03.007
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