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Ultrafast Protein Splicing is Common Among Cyanobacterial Split Inteins: Implications for Protein Engineering
We describe the first systematic study of a family of inteins, the split DnaE inteins from cyanobacteria. By measuring in vivo splicing efficiencies and in vitro kinetics, we demonstrate that several inteins can catalyze protein trans-splicing in tens of seconds, rather than hours, as is commonly ob...
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| Autors principals: | , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2012
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3535263/ https://ncbi.nlm.nih.gov/pubmed/22734434 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja303226x |
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