Molecular cloning of the Harvey sarcoma virus closed circular DNA intermediates: initial structural and biological characterization.

Supercoiled Harvey sarcoma virus (Ha-SV) DNA was extracted from newly infected cells by the Hirt procedure, enriched by preparative agarose gel electrophoresis, and digested with EcoRI, which cleaved the viral DNA at a unique site. The linearized Ha-SV DNA was then inserted into lambda gtWESlambda B...

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Hlavní autoři: Hager, G L, Chang, E H, Chan, H W, Garon, C F, Israel, M A, Martin, M A, Scolnick, E M, Lowy, D R
Médium: Artigo
Jazyk:Inglês
Vydáno: 1979
Témata:
Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC353507/
https://ncbi.nlm.nih.gov/pubmed/229252
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