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Molecular cloning of the Harvey sarcoma virus closed circular DNA intermediates: initial structural and biological characterization.

Supercoiled Harvey sarcoma virus (Ha-SV) DNA was extracted from newly infected cells by the Hirt procedure, enriched by preparative agarose gel electrophoresis, and digested with EcoRI, which cleaved the viral DNA at a unique site. The linearized Ha-SV DNA was then inserted into lambda gtWESlambda B...

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Udgivet i:J Virol
Principais autores: Hager, G L, Chang, E H, Chan, H W, Garon, C F, Israel, M A, Martin, M A, Scolnick, E M, Lowy, D R
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1979
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC353507/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/229252/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.31.3.795-809.1979
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