Polarization-resolved second-harmonic generation microscopy as a method to visualize protein-crystal domains

Polarization-resolved second-harmonic generation (PR-SHG) microscopy is described and applied to identify the presence of multiple crystallographic domains within protein-crystal conglomerates, which was confirmed by synchrotron X-ray diffraction. Principal component analysis (PCA) of PR-SHG images...

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Detalhes bibliográficos
Main Authors: DeWalt, Emma L., Begue, Victoria J., Ronau, Judith A., Sullivan, Shane Z., Das, Chittaranjan, Simpson, Garth J.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2013
Assuntos:
Research Papers
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3532131/
https://ncbi.nlm.nih.gov/pubmed/23275165
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S0907444912042503
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