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Protein-Aptamer Binding Studies Using Microchip Affinity Capillary Electrophoresis

The use of traditional capillary electrophoresis (CE) to detect weak binding complexes is problematic due to the fast-off rate resulting in the dissociation of the complex during the separation process. Additionally, proteins involved in binding interactions often nonspecifically stick to the bare-s...

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Bibliographic Details
Main Authors: Gong, Maojun, Nikcevic, Irena, Wehmeyer, Kenneth R., Limbach, Patrick A., Heineman, William R.
Format: Artigo
Language:Inglês
Published: 2008
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3529586/
https://ncbi.nlm.nih.gov/pubmed/18324729
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elps.200700777
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